Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803.
نویسندگان
چکیده
We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steady-state fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 79 8 شماره
صفحات -
تاریخ انتشار 2013